Students use a problem-based approach to gene cloning; for example, cloning the gene for an industrially important enzyme, a-amylase from Bacillus licheniformis. In this example, students would purify a-amylase from bacillus licheniformis and affinity-tagged a-amylase overproduction clone for purification and compare the activity of the purified proteins. Credit given for only one of BIOT-T315 or BIOL-T315.